QuantaCell extracts robust quantitative data from 2D, 3D and time-lapse microscopy images, from individual images to High-Content Screening experiments.

• Multi-scale 2D/3D segmentation & reconstruction — Detection and reconstruction of organoids, cells, nuclei and biological structures from 2D images and 3D image stacks.
• Time-lapse analysis & tracking — Tracking of cells, organoids and biological structures to quantify movement, growth and morphological changes over time.
• Morphological measurements — Quantification of volume, diameter, surface area, shape, compactness and structural heterogeneity.
• Fluorescence quantification & spatial measurements — Measurement of marker intensity and localization, as well as cell distribution and spatial organization.
• Multi-condition analysis & High-Content Screening — Quantitative comparison across wells, treatments, doses, time points and experimental groups.

Applications of QuantaCell’s 3D image analysis for organoids, spheroids and 3D tissue models.

• Cellular phenotyping and cell type classification
  Characterize cell and organoid populations based on morphology, marker expression and spatial organization in 2D or 3D.
  
• Treatment response assessment
  Quantify growth, morphological changes and phenotypic responses to treatments over time, including time-lapse experiments.
  
• Detection of biological structures
  Detect and quantify lumens, rosettes, crypt-like regions, necrotic cores and other structures of interest in 2D or 3D models.
  
•  Spatial biology & cell tracking
  Analyze cell localization, interactions and trajectories to characterize spatial organization and dynamic behavior.
• High-content screening & drug response
  Quantify phenotypic and dynamic readouts across plates, wells, compounds, doses and experimental conditions in 2D or 3D models.
• Patient-derived organoid analysis
  Quantify patient-specific phenotypes and treatment responses in patient-derived organoid models.