The AI-Powered organoids, spheroids, and 3D cell cultures quantification software
The most powerful 3D High Content Screening software on the market !
Segment then Analyze !
3D segmentation and high-content screening analysis software to navigate your assay and monitor drug effects.

AssayScope
The software for organoids and 3D cell cultures/tissues to simplify your 3D analyses.
After acquiring your 3D images, you will be able to:
• Accurately segment nuclei, cells, organoids, and 3D spheroids,
• Extract key features such as shapes and channels from 3D cell cultures,
• Visualize 3D tissue samples using surface or volumetric rendering,
• Generate and interpret sophisticated graphs and diagrams (population, z-factor, t-test, PCA, …),
• Navigate immersively between 3D images and graphs.
Examples of Applications
• Evaluate the precise impact of drug treatments on cell morphology and viability.
• Understand the formation and behavior of tumor microenvironments by observing and analyzing cell interactions within an organoid or spheroid.
• Develop more targeted and effective treatments by adapting 3D tissue analyses based on the characteristics of a patient’s cells for personalized medicine research.


Volumetric and surface visualization of a spheroid
“To equip researchers and biologists to tackle the challenges of personalized medicine,
we have developed an AI-enhanced 3D image analysis software.“
Why choose AssayScope?
3D SEGMENTATION
HCS ANALYSIS
Robust Nuclei Segmentation
Segment 3D nuclei in real life conditions, compatible with all image qualities, resolutions and dimensions

Visualization
Efficient 3D visualization: surface rendering, volume rendering
Cell and Organoid
Simple and efficient cell and organoid segmentations. Feature extraction (shapes and channels)
User Interactivity
Link between images and charts for immersive navigation and smooth data exploration
Plots and Statistics
Proves all charts and plots adapted to population analysis, z-factor, t-test, pca adapted to segmented 3D imaging

- Attractive launch offer
- Demo on your data
- Open for collaboration
Join the expanding community of researchers
using AssayScope to elevate their 3D analysis
AssayScope meets the needs of researchers and biologists
working with organoids, spheroids, and 3D tissues by enabling them to:
• Quickly and accurately segment nuclei, cells, and complex 3D structures.
• Analyze large series of images from high-content screening.
• Extract reliable quantitative measurements (size, shape, intensity, distribution, etc.).
• Visualize results in 3D to better understand biological structures.
• Directly link statistical data to source images.
• Identify and isolate cell subpopulations through interactive gating.
• Reduce reliance on lengthy and subjective manual analyses.
• Streamline your image analysis workflow while maintaining the reproducibility of results.
Segmentation of 3D nuclei
AssayScope integrates advanced segmentation algorithms based on artificial intelligence, optimized for nuclei, cells, organoids, and spheroids in 3D.
Features
- Robust AI algorithms trained on a wide variety of image types and experimental conditions.
- Compatibility with different acquisition resolutions and qualities.
- Segmentation adapted to simple or complex structures, even with noise or overlaps.
- Automated extraction of morphological and intensity measurements.
User experience
- Reliable results in just a few clicks, without complex settings.
- Immediate visualization of segmented masks in 3D.
- Reduction of bias associated with manual segmentations.
- Significant time savings in screening and analysis workflows.
3D Nuclei Segmentation Quality
Segmentation of 3D Cells
3D Cells Segmentation Quality
Segmentation of 3D organoids
Gating of cell populations with 3D image feedback
AssayScope features an interactive gating tool that allows you to easily select cell subpopulations directly from your analysis plots.
User experience
Smooth navigation between statistics and images.
Immediate understanding of the chosen population’s characteristics.
Considerable time savings in data exploration and interpretation.
Gating with 3D image feedback
Enhance 3D Analysis with AssayScope
Accelerate your research on organoids, spheroids, and 3D tissues using the power of AI.
Conduct 3D analysis of your results directly! No lengthy or complex training required.
Welcome to AssayScope, the high-content 3D segmentation and screening software designed to simplify the analysis of complex 3D cell culture images, organoids, and spheroids. With the most advanced artificial intelligence technology, AssayScope allows you to easily extract crucial information and visualize results instantly, without requiring extensive technical expertise. Effortlessly navigate through 3D datasets, segment and analyze cellular structures, and gain meaningful insights with just a few clicks.
Enjoy a cost-effective solution without the need for expensive equipment or intensive training programs. AssayScope optimizes your workflows and offers powerful analytical tools for 3D cell cultures, helping you save time and maximize resources.
Let’s explore the key features that make AssayScope the essential solution for your 3D analyses on organoids, spheroids, and 3D tissues. Research use only.
Key Strengths of AssayScope
High-Precision 3D Segmentation
AssayScope offers robust segmentation of nuclei, cells, organoids, and spheroids under real-world conditions, while remaining compatible with a wide range of image qualities, resolutions, and 3D tissue dimensions. This enables users to work with diverse samples.
Visualization
With its 3D visualization capabilities, AssayScope provides surface and volumetric renderings of organoids and spheroids, enhancing the visual understanding of these structures.
Advanced Analysis
Additionally, the graphical and statistical tools provided enable detailed analysis of AI-segmented 3D images, facilitating trend detection and performing specific analyses such as population studies, statistical tests (z-factor, t-test), and principal component analysis (PCA).
Immersive Navigation
One of AssayScope’s strengths is its interactive graphical interface, which creates a direct link between 3D cell culture images and graphs. This feature allows for seamless data exploration, making the user experience not only more enjoyable but also more efficient for analysis and decision-making.
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- Image analysis in patients studies
- Characterization of drug/compounds effects
- Automatic detection of rare events

Services of deep learning generation
- Creation of precise annotations for training
- Create powerfull DL models for 2D or 3D
- Optimize speed and performances

Software development for biomedical imaging
- Customized software solutions with ergonomics UIX
- AI for automatic analysis
- Industrialization of analyses in laboratories
Our Last Publications
Our methods and expertise are recognized by the scientific community
through collaborations and peer-reviewed publications

May 2025
With advanced AI and 3D imaging, we reveal organoid structure and dynamics across scales. From nuclei to full organoids, our analysis delivers actionable data for biomedical innovation.

Automated high-speed 3D imaging
July 2022
Our automated platform streamlines 3D organoid culture and imaging, enabling rapid multi-scale analysis. From single cells to entire structures, deep learning quantifies organoid organization with precision.

AI for pulmonary nodule classification
December 2020
By uniting deep learning and medical imaging, we deliver accurate pulmonary nodule detection and classification — achieving 89% sensitivity and 86% specificity to support earlier and more reliable diagnoses.
QuantaCell, Campus Balard, IHU
Contact
+33 (0) 9 83 33 81 90
300 av. du Pr Emile Jeanbrau
34090 Montpellier, France