Light microscopy for applications in cellular and histological biology
The QuantaCell team participates in many studies involving imaging, to answer various biological questions.
Biological imaging allows the visualization and quantification of biological organization at various levels, ranging from small animals, organs and cells, down to the molecular level. QuantaCell proposes guidance in your choice of instruments and their settings. The choice of equipment and materials is key to the quality of your experiments. The main parameters to take into account are:
Depends on the objective and camera
- If too low -> risk of rendering insufficient accuracy
- If too high -> risk of getting too many data to manage, and of loss of time (human resource and informatics)
- Useful for resolving the background noise in images by out-of-focus objects. Allows the increase of contrast.
- As the confocal microscopy is heavier in use, slower, and more expensive, it is preferable to use a wide field microscope when confocality is not a necessity.
- Color camera for natural colors (histological sections, phase contrast, …)
- Monochromatic camera for acquisition of fluorescent signals
- High sensitivity for short illuminations or low emissions (Quantum Dots, SPT)
- Many pixels for high spatial resolution with very rich details
The motorized stage
Allows the acquisition of many fields in a multi-well plate or whole glass slide
- The accuracy of the stage should be less than the size of objects to be analyzed
- The speed of the stage is a key determinant for acquisitions in video-microscopy.
Allows for culturing cells or organisms under optimal physiological conditions during microscopy. The parameters to be controlled are temperature, humidity and the carbon dioxide in air atmosphere
The plate or slide feeder
Allows for automatic sequential acquisition of multiple multi-well plates or slides
Modality of the microscope
Defines how the image is formed. Variation in modality allows for the acquisition of different characteristics of the samples
- FRAP/Photo Activation