Automatic detection of the growth of axons in microscopy of fluorescence
An explant of the medulla is cultivated in the presence of a chemical attractor. Directionality of the axon growth is studied. In the following illustration, the explant is labeled in GFP alpha-tubulin. Axons outside of the explant are automatically detected. Their direction is represented in the rose diagram, showing the influence of the chemical attractor, in a statistical, reproducible and unbiased manner.
Credit : Séverine Marcos et Evelyne Bloch-Gallego (Institut Cochin, Paris, France).
Reference : Tubulin Tyrosination Is Required for the Proper Organization and Pathfinding of the Growth Cone. Séverine Marcos, Julie Moreau, Stéphanie Backer, Didier Job, Annie Andrieux, Evelyne Bloch-Gallego, PLoS ONE 4, 4 (2009) e5405.